SARS-COV-2 Test

Txij li thaum lub Kaum Ob Hlis 2019, COVID-19 tshwm sim los ntawm tus mob hnyav ua pa nyuaj (SARS) tau kis thoob ntiaj teb.Tus kab mob ua rau COVID-19 yog SARS-COV-2, ib leeg-stranded ntxiv rau strand RNA tus kab mob uas yog cov tsev neeg tus mob coronaviruses.β coronaviruses yog spherical los yog oval nyob rau hauv cov duab, 60-120 nm inch, thiab feem ntau pleomorphic.Vim tias lub hnab ntawv ntawm tus kab mob muaj lub ntsej muag uas tuaj yeem txuas mus rau txhua sab thiab zoo li tus kab mob corolla, nws muaj npe hu ua coronavirus.Nws muaj cov tshuaj ntsiav, thiab S (Spike protein), M (Membrane protein), M (matrix protein) thiab E (Envelope protein) raug faib rau hauv cov tshuaj ntsiav.Lub hnab ntawv muaj RNA khi rau N (Nucleocapsid protein).Cov protein SSARS-COV-2muaj S1 thiab S2 subunits.Lub receptor-binding domain (RBD) ntawm S1 subunit induces SARS-COV-2 kab mob los ntawm kev khi rau angiotensin converting enzyme 2 (ACE2) ntawm lub xov tooj ntawm tes.

Sars-cov-2 tuaj yeem kis tau los ntawm ib tus neeg mus rau lwm tus thiab kis tau ntau dua li sarS-COV, uas tau tshwm sim xyoo 2003. Nws kis tau los ntawm cov pa ua pa thiab ze rau tib neeg, thiab tuaj yeem kis tau los ntawm aerosol yog tias muaj nyob hauv ib puag ncig. nrog zoo airtight rau lub sijhawm ntev.Cov tib neeg feem ntau kis tau tus kab mob, thiab lub sij hawm incubation yog 1 mus rau 14 hnub, feem ntau yog 3 mus rau 3 hnub.Tom qab kis tus kabmob tshiab, cov mob me me ntawm COVID-19 yuav tsim cov tsos mob feem ntau yog ua npaws thiab hnoos qhuav.COVID-19 yog kis tau zoo heev thiab kis tau zoo heev hauv cov theem asymptomatic ntawm kev kis kab mob.Tus kab mob Sars-cov-2 tuaj yeem ua rau kub taub hau, hnoos qhuav, qaug zog thiab lwm yam tsos mob.Cov neeg mob hnyav feem ntau tshwm sim dyspnea thiab / lossis hypoxemia 1 lub lis piam tom qab pib, thiab cov neeg mob hnyav tuaj yeem ua rau mob ua pa nyuaj, coagulopathy thiab ntau lub cev tsis ua haujlwm.

Vim tias sarS-COV-2 yog kis tau zoo heev thiab ua rau tuag taus, sai, raug thiab yooj yim kev kuaj mob rau kev kuaj pom tus kab mob SARS-COV-2 thiab cais cov neeg muaj tus kab mob (xws li cov neeg muaj mob asymptomatic) yog tus yuam sij rau kev tshawb pom qhov kis kab mob, thaiv cov kab mob. kis kab mob ntawm cov kab mob thiab tiv thaiv thiab tswj kev sib kis.

POCT, tseem hu ua bedside detection technology los yog real-time detection technology, yog ib hom kev tshawb nrhiav uas yog ua nyob rau hauv sampling site thiab tuaj yeem nrhiav tau sai sai los ntawm kev siv portable analytical instruments.Nyob rau hauv cov nqe lus ntawm kev kuaj kab mob, POCT muaj qhov zoo ntawm kev tshawb nrhiav ceev ceev thiab tsis muaj qhov chaw txwv piv nrog cov kev tshawb pom ib txwm muaj.POCT tsis tuaj yeem ua kom nrawm ntawm kev tshawb pom ntawm COVID-19, tab sis kuj zam kev sib cuag ntawm cov neeg ua haujlwm tshawb nrhiav thiab cov neeg mob thiab txo qis kev pheej hmoo kis mob.Tam sim no,Kev kuaj mob COVID-19cov chaw hauv Suav teb feem ntau yog cov tsev kho mob thiab cov chaw sim thib peb, thiab cov neeg ua haujlwm kuaj xyuas yuav tsum tau coj mus kuaj ncaj qha rau pem hauv ntej ntawm tib neeg los kuaj.Txawm hais tias muaj kev tiv thaiv, kev coj mus kuaj ncaj qha los ntawm tus neeg mob ua rau muaj kev pheej hmoo kis mob rau tus neeg kuaj nws.Yog li ntawd, peb lub tuam txhab tau tsim tshwj xeeb tsim cov khoom siv rau cov neeg coj mus kuaj hauv tsev, uas muaj qhov zoo ntawm kev tshawb pom sai, kev ua haujlwm yooj yim, thiab kev tshawb nrhiav hauv tsev, chaw nres tsheb thiab lwm qhov chaw uas tsis muaj kev tiv thaiv biosafety.

Cov thev naus laus zis tseem ceeb siv yog immunochromatography thev naus laus zis, tseem hu ua Lateral Flow assay (LFA), uas yog txoj kev tshawb pom sai sai uas tau tsav los ntawm kev ua haujlwm capillary.Raws li ib tug kuj paub tab paub ceev ceev technology, nws muaj ib tug yooj yim kev khiav hauj lwm, luv luv cov tshuaj tiv thaiv lub sij hawm thiab ruaj khov.Tus neeg sawv cev ib qho yog colloidal kub immunochromatography ntawv (GLFA), uas feem ntau suav nrog cov qauv ncoo, daim ntawv cog lus ncoo, nitrocellulose (NC) zaj duab xis thiab dej nqus ncoo, thiab lwm yam. Cov ntaub ntawv cog lus yog tsau nrog cov tshuaj tiv thaiv kev hloov pauv kub nanoparticles (AuNPs), thiab NC. zaj duab xis yog tsau nrog capture antibody.Tom qab cov qauv ntxiv rau hauv cov qauv ncoo, nws ntws los ntawm cov ntaub ntawv sib txuas thiab NC zaj duab xis ua tiav raws li qhov kev txiav txim ntawm capillary, thiab thaum kawg mus txog lub ncoo absorbent.Thaum cov qauv ntws los ntawm cov ntaub ntawv khi, cov khoom yuav tsum tau ntsuas hauv cov qauv yuav khi nrog cov cim kub antibody;Thaum cov qauv ntws los ntawm NC daim nyias nyias, cov qauv uas yuav tsum tau kuaj tau raug ntes thiab kho los ntawm cov tshuaj tua kab mob, thiab cov kab liab liab tshwm rau ntawm NC daim nyias nyias vim muaj cov khoom kub nanoparticles.Kev kuaj pom sai sai ntawm SARS-COV-2 tuaj yeem ua tiav los ntawm kev soj ntsuam cov kab liab hauv thaj chaw kuaj pom.Cov khoom siv ntawm txoj kev no yog ib qho yooj yim rau kev lag luam thiab tus qauv, yooj yim rau kev khiav lag luam thiab ceev teb.Nws yog qhov tsim nyog rau kev tshuaj xyuas cov pej xeem loj thiab siv dav hauv kev tshawb nrhiav tus kabmob tshiab.

Novel kab mob coronavirusyog ib qho kev sib tw loj heev uas ntsib lub ntiaj teb no.Kev kuaj mob sai thiab kho raws sijhawm yog tus yuam sij rau kev yeej kev sib ntaus sib tua.Nyob rau hauv lub ntsej muag ntawm cov kab mob siab thiab cov neeg muaj tus kab mob ntau, nws yog ib qho tseem ceeb heev los tsim cov khoom siv kom raug thiab ceev ceev.Nws paub tias ntawm cov qauv siv feem ntau, alveolar lavage kua muaj qhov zoo tshaj plaws ntawm cov pharyngeal swabs, qaub ncaug, hnoos qeev thiab alveolar lavage kua.Tam sim no, qhov kev sim ntau tshaj plaws yog coj mus kuaj los ntawm cov neeg mob uas xav tias mob caj pas los ntawm lub pharynx sab sauv, tsis yog qhov ua pa hauv qab, qhov twg tus kab mob tuaj yeem nkag tau yooj yim.Tus kab mob no tseem tuaj yeem kuaj pom hauv cov ntshav, zis, thiab quav, tab sis nws tsis yog qhov chaw tseem ceeb ntawm kev kis tus kab mob, yog li tus kab mob no tsawg thiab tsis tuaj yeem siv los kuaj xyuas.Tsis tas li ntawd, raws li RNA tsis ruaj khov thiab yooj yim rau degrade, kev kho kom tsim nyog thiab kev rho tawm cov qauv tom qab sau kuj yog qhov tseem ceeb.

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